Nanobind Tissue Big DNA Kit

SKU NB-900-701-01
$450.00
In stock
1
Product Details

Sequencing a Fish? A Monkey? A Shark? A Crab? Human Tissues?

The Nanobind Tissue Big DNA Kit is designed for rapid extraction of really big DNA from diverse tissues including mammalian, fish, avian, mollusk, and crustacean samples.

This kit has been used to sequence everything from pigs to sea slugs, king crabs, giant prawns, zebra finch, and Antarctic ice fish.

The kit can also be used to run any of the Nanobind CBB Big DNA Kit protocols including cultured cells, bacteria, and blood.

Includes protocols for high MW DNA (50 kb - 300+ kb) extraction.

Generates consistent read length N50 from 20 - 60 kb from nearly any sample type on any platform.

Application Notes are provided to cover many specific sequencing applications.

All protocols have been thoroughly validated on platforms including: 1) PacBio Sequel I/II, 2) Oxford Nanopore MinION/GridION/PromethION, 3) 10X Genomics Chromium and 4) any other application high quality DNA is needed.

Typical yields are 5 ug - 100+ ug per extraction depending on tissue type and input.

Insect samples are not officially supported at this time with the Tissue Kit. However, select insects can be processed with supplemental buffers. Contact us for more details.

Worm samples are not officially supported at this time with the Tissue Kit. However, select worms can be processed with supplemental buffers. Contact us for more details.

See our publications page for latest results.

We will make every effort to help you get big DNA!


Includes:

1) Proteinase K, 2) RNase A, 3) Buffer CT, 4) Buffer SB, 5) CLE3 Digestion Buffer, 6) BL3 Lysis/Binding Buffer, 7) CW1 Wash Buffer, 8) CW2 Wash Buffer, 9) EB Elution Buffer, and 10) 20x MagNanobind Disks.

20 extractions per kit.


Protocols

Nanobind Tissue Big DNA Kit - Handbook v1.0


Application Notes

cryoPREP Tissue Homogenization Application Note v1

Human Breast Application Note v1

Mammalian Brain Application Note v1

Mammalian Liver Application Note v1

Mammalian Spleen Application Note v1

Fish Testis Application Note v1

Snail Application Note v1

Crab Application Note v1

Fish Skeletal Muscle Application Note v1

Nucleated Blood Application Note v1

Fish Tissue Sequencing Guide for ONT v1

Aplysia Application Note v1

DNA Loading Sequencing Guide for ONT v1


Version History

Nanobind Big DNA Kit - Version History


Safety

SDS


Legal

Patents / Terms and Conditions


International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.


We'd love to chat about sample prep and long-reads (info@circulomics.com)!

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701 E Pratt St, Baltimore, MD 21202

INFO@CIRCULOMICS.COM

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