Sequencing a Fish? A Monkey? A Shark? A Crab? Human Tissues?
The Nanobind Tissue Big DNA Kit is designed for rapid extraction of really big DNA from diverse tissues including mammalian, fish, avian, mollusk, and crustacean samples.
This kit has been used to sequence everything from pigs to sea slugs, king crabs, giant prawns, zebra finch, and Antarctic ice fish.
The kit can also be used to run any of the Nanobind CBB Big DNA Kit protocols including cultured cells, bacteria, and blood.
Includes protocols for high MW DNA (50 kb - 300+ kb) extraction.
Generates consistent read length N50 from 20 - 60 kb from nearly any sample type on any platform.
Application Notes are provided to cover many specific sequencing applications.
All protocols have been thoroughly validated on platforms including: 1) PacBio Sequel I/II, 2) Oxford Nanopore MinION/GridION/PromethION, 3) 10X Genomics Chromium and 4) any other application high quality DNA is needed.
Typical yields are 5 ug - 100+ ug per extraction depending on tissue type and input.
Insect samples are not officially supported at this time with the Tissue Kit. However, select insects can be processed with supplemental buffers. Contact us for more details.
Worm samples are not officially supported at this time with the Tissue Kit. However, select worms can be processed with supplemental buffers. Contact us for more details.