Nanobind Tissue Big DNA Kit

Sequencing a Fish? A Monkey? A Shark? A Crab? Human Tissues?

The Nanobind Tissue Big DNA Kit is designed for rapid extraction of really big DNA from diverse animal tissues including mammalian, fish, avian, mollusk, and crustacean samples.

Our Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.

This kit has been used to sequence everything from pigs to sea slugs, king crabs, giant prawns, zebra finch, and Antarctic ice fish.

The kit can also be used to run any of the Nanobind CBB Big DNA Kit protocols including cultured cells, bacteria, and blood.

Includes protocols for HMW DNA (50 kb - 300+ kb) and UHMW DNA (50 kb - 1+ Mb) extraction.

Generates consistent read length N50 from 20 - 60 kb from nearly any sample type on any platform.

Read length N50 up to 100 kb can be obtained using ultra long sequencing.

Application Notes are provided to cover many specific sequencing applications.

All protocols have been thoroughly validated on platforms including: 1) PacBio Sequel I/II, 2) Oxford Nanopore MinION/GridION/PromethION, 3) 10X Genomics Chromium and 4) any other application high quality DNA is needed.

Typical yields are 5 ug - 100+ ug per extraction depending on tissue type and input.

Insect samples are not officially supported at this time. However, select insects can be processed with supplemental buffers. Contact us for more details.

Worm samples are not officially supported at this time. However, select worms can be processed with supplemental buffers. Contact us for more details.

Fungal samples are not officially supported at this time. However, select fungal can be processed with supplemental buffers. Contact us for more details.

See our publications page for latest results.

We will make every effort to help you get big DNA!

Includes:

1) Proteinase K, 2) RNase A, 3) Buffer CT, 4) Buffer SB, 5) CLE3 Digestion Buffer, 6) BL3 Lysis/Binding Buffer, 7) CW1 Wash Buffer, 8) CW2 Wash Buffer, 9) EB Elution Buffer, and 10) 20x Nanobind Disks.

20 extractions per kit.

Protocols

Nanobind Tissue Big DNA Kit Handbook

See Nanobind Support page for full list.

Application Notes

cryoPREP Tissue Homogenization Application Note v1

Human Breast Application Note v1

Mammalian Brain Application Note v1

Mammalian Liver Application Note v1

Mammalian Spleen Application Note v1

Fish Testis Application Note v1

Snail Application Note v1

Crab Application Note v1

Fish Skeletal Muscle Application Note v1

Nucleated Blood Application Note v1

Fish Tissue Sequencing Guide for ONT v1

Aplysia Application Note v1

DNA Loading Sequencing Guide for ONT v1

Safety

SDS

All Kits are Guaranteed for 1 Year from the Date of Purchase.

Legal

Patents / Terms and Conditions

International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.

We'd love to chat about sample prep and long-reads (support@pacb.com)!