HMW DNA extraction from cells, bacteria, and blood (24 reactions)
The Nanobind CBB kit is designed for rapid extraction of large DNA from cultured cells, cultured bacteria (gram-negative and gram-positive), and whole blood (nucleated and non-nucleated).
Our Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.
Read length N50 of 30–50 kb can be expected in standard sequencing applications but N50 in excess of 150 kb can be achieved using ultra-long sequencing protocols.
Combine with the SRE kits to further enhance reads lengths.
Nanobind HMW DNA works well on platforms including: 1) PacBio RSII/Sequel/Sequel II and IIe; 2) Oxford Nanopore MinION/GridION/PromethION; 3) Bionano Genomics Irys/Saphyr; 4) 10X Genomics Chromium; and 5) any other application where high-quality DNA is needed.
Includes protocols for HMW DNA (50 kb–300+ kb) and UHMW DNA (50 kb–1 Mb+) extraction.
Typical yields are 5–100+ µg per extraction depending on input.
This kit has not been validated for complex sample types.
The Nanobind CBB kit is comprised of the Nanobind CBB kit RT (102-207-600) and Nanobind CBB kit 4C (102-207-700). Components from both kits are required to complete HMW DNA extraction from cells, bacteria, and blood (24 reactions per kit).
Gram-positive bacteria require user-supplied lysozyme and STET buffer.