We've generated the longest sequencing read ever! 2.44 Mb on Oxford Nanopore MinION using Nanobind extracted UHMW DNA. Protocol coming soon!
Enter the contest to win some Nanobind and SRE Kits as well as some nifty Circulomics scarves.
Have a Tough Animal to Sequence? The Nanobind Tissue Big DNA Kit is Here! Use Short Read Eliminator to Deplete Small DNA.
We use micro-, nano-, and single molecule technologies to streamline every step in the genomics workflow from sample preparation to assays and detection instruments.
Nanobind magnetic disks use silica nanotechnology and a rapid bind, wash, and elute process to provide the highest quality nucleic acids and the highest yields. High molecular weight (HMW) and ultra high molecular weight (UHMW) DNA for Oxford Nanopore and PacBio sequencing and Bionano optical mapping. Compatible with manual or automated processing. Other applications including NGS size selection, FFPE, swabs, RNA, and large volume plasmid preps are in the works.
Nanobind Big DNA Kits are available now in the Store.
Micro and Nanoscale SilicaEach non-porous Nanobind disk is covered with a high density of micro- and nanostructured silica that can bind vast amounts of DNA and protect it from shearing. A single disk is used per extraction and can be processed either manually or automated using standard robotic instruments.
Rapid Bind, Wash, and EluteHMW DNA extraction can be performed using a simple bind, wash, and elute protocol that is automation compatible.
HMW DNA (50 - 300+kb)Nanobind Big DNA Kits can be used to obtain HMW DNA (50 - 300+ kb) from nearly all sample types. All samples have been validated by long-read sequencing on PacBio and ONT.
UHMW DNA (50 kb - 1+ Mb)Nanobind's High Molecular Weight (HMW) process provides DNA from 50 kb - 300 kb while the Ultra High Molecular Weight (UHMW) process provides DNA from 50 kb - 5.7 Mb+.
Ultra Long Reads on ONT (2+ Mb)Nanobind UHMW DNA and Nanobind Reaction Cleanup were used to generate the longest sequencing read ever, a 2.44 Mb length read from human blood. Ultra long reads (1 - 2+ Mb) can also be generated from cultured cells and bacteria.
Cells, Bacteria, and BloodThe Nanobind CBB Big DNA Kit can be used to extract HMW and UHMW DNA from cultured cells, cultured bacteria, and whole blood.
PlantsThe Nanobind Plant Nuclei Big DNA Kit can be used to extract HMW DNA from a wide array of plants from pine needles and cotton to lavender and grapevines.
TissuesThe Nanbind Tissue Big DNA Kit can be used to extract HMW DNA from human, mouse, monkey, pig, fish, crab, snail, slug, bird, and select insect samples.
ONT SequencingNanobind Big DNA Kits generate consistent nanopore data across a variety of sample types including cells, blood, bacteria, plants, and tissues.
Enhance long-read sequencing read lengths by depleting short DNA. Short Read Eliminator Kits use a simple, 1 hour, size selective precipitation method. Three versions are available for DNA of all quality from amplicons to high quality HMW DNA. Protocols available for Oxford Nanopore MinION / GridION / PromethION. Protocols coming soon for PacBio Sequel I/II.
Short Read Eliminator Kits are available now in the Store.
3 Depletion CutoffsChoose your SRE kit based on the quality of your input DNA from fragmented DNA or PCR amplicons to high quality HMW DNA.
SRE XS Kit - Up to 10 kbShort DNA depletion begins at 10 kb and increases in effectiveness as the DNA gets shorter.
SRE Kit - Up to 25 kbShort DNA depletion begins at 25 kb and increases in effectiveness as the DNA gets shorter.
SRE XL Kit - Up to 40 kbShort DNA depletion begins at 40 kb and increases in effectiveness as the DNA gets shorter.
SRE XL on Nanobind Extracted Blood DNASRE XL begins depleting short DNA at 40 kb, leading to a 14 kb enhancement in read length N50.
Use single molecule analysis to better characterize your samples before costly sequencing or critical studies.
PicoSep Single Molecule SystemEach PicoSep system consists of a single molecule detection module (shown), electronics module, and flow control module. The entire system is USB-controlled by laptop.
PicoSep Separation CapillarySeparation is performed by injecting a sample plug into a buffer-filled microcapillary and driving with pressure. Single molecule sensitivity enables analysis to be performed using only 5 pL of sample and <20 DNA molecules.
PicoSep Single Molecule DNA SizingDNA molecules are individually detected (bottom) and counted to form chromatograms (top). Each vertical spike is a single DNA molecule. Larger molecules elute first.
PicoSep RNA Integrity AnalysisSingle molecule sensitivity enables detection of minute amounts of RNA degradation and more accurate quantification of RNA integrity by direct counting of degraded molecules.
PicoSep High MW DNA SizingPicoSep can accurately size and quantify high molecular weight DNA (100 kb+) in <2 hours. No electrophoresis method offers this speed, sizing resolution, or quantification capability.
We’re always looking for smart, dedicated, and creative people to join our team. As in any startup environment, candidates who are flexible enough to fill many roles can be expected to grow quickly within the company and play a large part in shaping its future. Today, you may be working at the lab bench while tomorrow, you may be writing a patent, and the day after, you may be meeting with customers.
Circulomics is a Maryland biotechnology company located within IMET's Harbor Launch Incubator. To date, Circulomics has received over $9M in funding from NIH and Maryland TEDCO to develop innovative platforms for nucleic acid sample preparation, long-read sequencing, nucleic acid assays, and single molecule analysis that will drive the research and clinical translation of genomics technologies from sequencing to expression profiling and biomarker analysis.
701 E Pratt St, Baltimore, MD 21202
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