80+ Customer Papers in 2020! See How Nanobind and Short Read Eliminator Have Been Used to Sequence Everything From Cells, Bacteria, and Fungi to Plants, Insects, Fish, Mammals and Reptiles.
The Nanobind UL Library Prep Kit is Available for Pre-Order. Reads Up to 4 Mb in Length! See Poster #30 at ONT NCM or the product page for Full Details. Visit Us At Upcoming Events!
Have a Tough Animal to Sequence? The Nanobind Tissue Big DNA Kit is Here! Use Short Read Eliminator to Deplete Small DNA.
We use micro-, nano-, and single molecule technologies to streamline every step in the genomics workflow from sample preparation to assays and detection instruments.
Nanobind magnetic disks are covered with nanoscale wrinkles and folds that protect DNA from shearing to generate longer reads across a broader range of sample types than any other technology.
Extract high molecular weight (HMW = 50 kb - 300+ kb) and ultra high molecular weight (UHMW = 50 kb - 1+ Mb) DNA using a rapid bind, wash, and elute protocol. Compatible with manual or fully automated processing.
From simple samples such as cells, bacteria, and blood to challenging samples such as animal tissues, insects, fish, fungi, and plants, nearly all have been sequenced using Nanobind. Thoroughly validated on Oxford Nanopore, PacBio, and Bionano Genomics.
Nanobind Big DNA Kits are available now in the Store.
Generate ultra long, megabase reads on MinION, GridION, and PromethION.
Micro and Nanoscale SilicaEach non-porous Nanobind disk is covered with a high density of micro- and nanostructured silica that can bind vast amounts of DNA and protect it from shearing. A single disk is used per extraction and can be processed either manually or automated using standard robotic instruments.
Rapid Bind, Wash, and EluteHMW DNA extraction can be performed using a simple bind, wash, and elute protocol that is automation compatible.
HMW DNA (50 - 300+kb)Nanobind Big DNA Kits can be used to obtain HMW DNA (50 - 300+ kb) from nearly all sample types. All samples have been validated by long-read sequencing on PacBio and ONT.
UHMW DNA (50 kb - 1+ Mb)Nanobind's High Molecular Weight (HMW) process provides DNA from 50 kb - 300 kb while the Ultra High Molecular Weight (UHMW) process provides DNA from 50 kb - 5.7 Mb+.
Ultra Long Reads on ONT (4+ Mb)Nanobind UHMW DNA Extraction and Nanobind UL Library Prep were used to generate the longest sequencing read ever, a 4.1 Mb length read from human cells. Ultra long reads (1 - 2+ Mb) can also be generated from blood, bacteria, animal tissues and plants.
Cells, Bacteria, and BloodThe Nanobind CBB Big DNA Kit can be used to extract HMW and UHMW DNA from cultured cells, cultured bacteria, and whole blood.
PlantsThe Nanobind CBB Big DNA Kit can be used to extract HMW and UHMW DNA from cultured cells, cultured bacteria, and whole blood.
TissuesThe Nanbind Tissue Big DNA Kit can be used to extract HMW DNA from human, mouse, monkey, pig, fish, crab, snail, slug, bird, and select insect samples.
ONT SequencingNanobind Big DNA Kits generate consistent nanopore data across a variety of sample types including cells, blood, bacteria, plants, and tissues.
Enhance long-read sequencing read lengths by depleting short DNA.
Short Read Eliminator Kits use a simple, 1 hour, size selective precipitation method. Three versions are available for DNA of all quality from amplicons to high quality HMW DNA.
Protocols available for Oxford Nanopore MinION / GridION / PromethION. Protocols coming soon for PacBio Sequel I/II.
Short Read Eliminator Kits are available now in the Store.
3 Depletion CutoffsChoose your SRE kit based on the quality of your input DNA from fragmented DNA or PCR amplicons to high quality HMW DNA.
SRE XS Kit - Up to 10 kbShort DNA depletion begins at 10 kb and increases in effectiveness as the DNA gets shorter.
SRE Kit - Up to 25 kbShort DNA depletion begins at 25 kb and increases in effectiveness as the DNA gets shorter.
SRE XL Kit - Up to 40 kbShort DNA depletion begins at 40 kb and increases in effectiveness as the DNA gets shorter.
SRE XL on Nanobind Extracted Blood DNASRE XL begins depleting short DNA at 40 kb, leading to a 14 kb enhancement in read length N50.
Use single molecule analysis to better characterize your samples before costly sequencing or critical studies.
PicoSep Single Molecule SystemEach PicoSep system consists of a single molecule detection module (shown), electronics module, and flow control module. The entire system is USB-controlled by laptop.
PicoSep Separation CapillarySeparation is performed by injecting a sample plug into a buffer-filled microcapillary and driving with pressure. Single molecule sensitivity enables analysis to be performed using only 5 pL of sample and <20 DNA molecules.
PicoSep Single Molecule DNA SizingDNA molecules are individually detected (bottom) and counted to form chromatograms (top). Each vertical spike is a single DNA molecule. Larger molecules elute first.
PicoSep RNA Integrity AnalysisSingle molecule sensitivity enables detection of minute amounts of RNA degradation and more accurate quantification of RNA integrity by direct counting of degraded molecules.
PicoSep High MW DNA SizingPicoSep can accurately size and quantify high molecular weight DNA (100 kb+) in <2 hours. No electrophoresis method offers this speed, sizing resolution, or quantification capability.