Micro and Nanoscale SilicaEach non-porous Nanobind disk is covered with a high density of micro- and nanostructured silica that can bind vast amounts of DNA and protect it from shearing. A single disk is used per extraction and can be processed either manually or automated using standard robotic instruments.
Rapid Bind, Wash, and EluteHMW DNA extraction can be performed using a simple bind, wash, and elute protocol that is automation compatible.
HMW DNA (50 - 300+kb)Nanobind Big DNA Kits can be used to obtain HMW DNA (50 - 300+ kb) from nearly all sample types. All samples have been validated by long-read sequencing on PacBio and ONT.
UHMW DNA (50 kb - 1+ Mb)Nanobind's High Molecular Weight (HMW) process provides DNA from 50 kb - 300 kb while the Ultra High Molecular Weight (UHMW) process provides DNA from 50 kb - 5.7 Mb+.
Ultra Long Reads on ONTNanobind UHMW DNA can be used to obtain megabase length reads on Oxford Nanopore and for optical mapping.
Cells, Bacteria, and BloodThe Nanobind CBB Big DNA Kit can be used to extract HMW and UHMW DNA from cultured cells, cultured bacteria, and whole blood.
PlantsThe Nanobind Plant Nuclei Big DNA Kit can be used to extract HMW DNA from a wide array of plants from pine needles and cotton to lavender and grapevines.
TissuesThe Nanbind Tissue Big DNA Kit can be used to extract HMW DNA from human, mouse, monkey, pig, fish, crab, snail, slug, bird, and select insect samples.
3 Depletion CutoffsChoose your SRE kit based on the quality of your input DNA from fragmented DNA or PCR amplicons to high quality HMW DNA.
SRE XS Kit - Up to 10 kbShort DNA depletion begins at 10 kb and increases in effectiveness as the DNA gets shorter.
SRE Kit - Up to 25 kbShort DNA depletion begins at 25 kb and increases in effectiveness as the DNA gets shorter.
SRE XL Kit - Up to 40 kbShort DNA depletion begins at 40 kb and increases in effectiveness as the DNA gets shorter.
Nanobind Blood DNA SRE XLSRE XL begins depleting short DNA at 40 kb, leading to a 14 kb enhancement in read length N50.
PicoSep Single Molecule SystemEach PicoSep system consists of a single molecule detection module (shown), electronics module, and flow control module. The entire system is USB-controlled by laptop.
PicoSep Separation CapillarySeparation is performed by injecting a sample plug into a buffer-filled microcapillary and driving with pressure. Single molecule sensitivity enables analysis to be performed using only 5 pL of sample and <20 DNA molecules.
PicoSep Single Molecule DNA SizingDNA molecules are individually detected (bottom) and counted to form chromatograms (top). Each vertical spike is a single DNA molecule. Larger molecules elute first.
PicoSep RNA Integrity AnalysisSingle molecule sensitivity enables detection of minute amounts of RNA degradation and more accurate quantification of RNA integrity by direct counting of degraded molecules.
PicoSep High MW DNA SizingPicoSep can accurately size and quantify high molecular weight DNA (100 kb+) in <2 hours. No electrophoresis method offers this speed, sizing resolution, or quantification capability.