Small Technologies

We've generated the longest sequencing read ever! 2.44 Mb on Oxford Nanopore MinION using Nanobind extracted UHMW DNA. Protocol coming soon!
Enter the contest to win some Nanobind and SRE Kits as well as some nifty Circulomics scarves.
Have a Tough Animal to Sequence? The Nanobind Tissue Big DNA Kit is Here! Use Short Read Eliminator to Deplete Small DNA.
Big Advances
 

News, Events, & Publications

Visit our Twitter feed for latest updates

See Circulomics at:

ASHG-Meeting-2020.png

October 2020

Booth #1045

ASHG 2020

AGBT.png

Feb 2020

Poster #409 - HMW DNA from Tissues /

Poster #202 - Automated HMW DNA 

AGBT 2020

ASHG2019.png

Oct 2019

High Throughput HMW DNA Extraction from Human Tissues

ASHG 2019

Logo-ABRF.png

Mar 2019

HMW DNA Extraction and Size Selection

 / PacBio Microbial Multiplexing

ABRF 2019

jax.png

June 2020 - Postponed to 2021

Sponsor / Talk - Generating Ultra Long Reads on Oxford Nanopore

JAX Long-Read Workshop

PAGLogo.jpg

Jan 2020

Booth #203 / Poster #113 - HMW DNA from Animals and Plants

PAG XXVIII

logo-g10k.jpg

Aug 2019

Sample Prep Workshop

Nanobind HMW DNA Extraction

Genome 10K Conference

PAGXXVII.png

Jan 2019

Rapid HMW DNA Extraction from Plant, Insect, Cell, and Tissue Samples

PAG XXVII

Logo-ABRF.png

Mar 2020

Booth #235 / Talk - Generating Ultra Long Reads On Oxford Nanopore

ABRF 2020

ONTevents.jpg

Dec 2019

Delegates

ONT NCM

Logo-NHGRI.jpg

May 2019

HMW DNA Extraction and Size Selection for Long Read Sequencing

NHGRI Advanced Genomics

AGBT.png

Feb 2019

Rapid Extraction and Size Selection of HMW DNA for Long-Read Sequencing

AGBT 2019

 

Our Technologies

We use micro-, nano-, and single molecule technologies to streamline every step in the genomics workflow from sample preparation to assays and detection instruments.

Nanobind magnetic disks use silica nanotechnology and a rapid bind, wash, and elute process to provide the highest quality nucleic acids and the highest yields. High molecular weight (HMW) and ultra high molecular weight (UHMW) DNA for Oxford Nanopore and PacBio sequencing and Bionano optical mapping. Compatible with manual or automated processing. Other applications including NGS size selection, FFPE, swabs, RNA, and large volume plasmid preps are in the works.

Nanobind Big DNA Kits are available now in the Store.

Micro and Nanoscale Silica
Each non-porous Nanobind disk is covered with a high density of micro- and nanostructured silica that can bind vast amounts of DNA and protect it from shearing. A single disk is used per extraction and can be processed either manually or automated using standard robotic instruments.
Rapid Bind, Wash, and Elute
HMW DNA extraction can be performed using a simple bind, wash, and elute protocol that is automation compatible.
HMW DNA (50 - 300+kb)
Nanobind Big DNA Kits can be used to obtain HMW DNA (50 - 300+ kb) from nearly all sample types. All samples have been validated by long-read sequencing on PacBio and ONT.
UHMW DNA (50 kb - 1+ Mb)
Nanobind's High Molecular Weight (HMW) process provides DNA from 50 kb - 300 kb while the Ultra High Molecular Weight (UHMW) process provides DNA from 50 kb - 5.7 Mb+.
Ultra Long Reads on ONT (2+ Mb)
Nanobind UHMW DNA and Nanobind Reaction Cleanup were used to generate the longest sequencing read ever, a 2.44 Mb length read from human blood. Ultra long reads (1 - 2+ Mb) can also be generated from cultured cells and bacteria.
Cells, Bacteria, and Blood
The Nanobind CBB Big DNA Kit can be used to extract HMW and UHMW DNA from cultured cells, cultured bacteria, and whole blood.
Plants
The Nanobind Plant Nuclei Big DNA Kit can be used to extract HMW DNA from a wide array of plants from pine needles and cotton to lavender and grapevines.
Tissues
The Nanbind Tissue Big DNA Kit can be used to extract HMW DNA from human, mouse, monkey, pig, fish, crab, snail, slug, bird, and select insect samples.
ONT Sequencing
Nanobind Big DNA Kits generate consistent nanopore data across a variety of sample types including cells, blood, bacteria, plants, and tissues.
PacBio Sequencing
Nanobind Big DNA Kits have been used to sequence a comprehensive variety of sample types on PacBio Sequel.
Show More
 
 

Enhance long-read sequencing read lengths by depleting short DNA. Short Read Eliminator Kits use a simple, 1 hour, size selective precipitation method. Three versions are available for DNA of all quality from amplicons to high quality HMW DNA. Protocols available for Oxford Nanopore MinION / GridION / PromethION. Protocols coming soon for PacBio Sequel I/II.

Short Read Eliminator Kits are available now in the Store.

3 Depletion Cutoffs
Choose your SRE kit based on the quality of your input DNA from fragmented DNA or PCR amplicons to high quality HMW DNA.
SRE XS Kit - Up to 10 kb
Short DNA depletion begins at 10 kb and increases in effectiveness as the DNA gets shorter.
SRE Kit - Up to 25 kb
Short DNA depletion begins at 25 kb and increases in effectiveness as the DNA gets shorter.
SRE XL Kit - Up to 40 kb
Short DNA depletion begins at 40 kb and increases in effectiveness as the DNA gets shorter.
Simple Precipitation Process
Process up to 20 samples in 1 hour with just a centrifuge.
Enhance Read Lengths
The read length enhancement scales with more aggressive depletion of short DNA.
SRE XL on Nanobind Extracted Blood DNA
SRE XL begins depleting short DNA at 40 kb, leading to a 14 kb enhancement in read length N50.
SRE on Puregene Extracted Cell DNA
SRE is compatible with DNA extracted using any technology.
Show More
 

Use single molecule analysis to better characterize your samples before costly sequencing or critical studies.

PicoSep Single Molecule System
Each PicoSep system consists of a single molecule detection module (shown), electronics module, and flow control module. The entire system is USB-controlled by laptop.
PicoSep Separation Capillary
Separation is performed by injecting a sample plug into a buffer-filled microcapillary and driving with pressure. Single molecule sensitivity enables analysis to be performed using only 5 pL of sample and <20 DNA molecules.
PicoSep Single Molecule DNA Sizing
DNA molecules are individually detected (bottom) and counted to form chromatograms (top). Each vertical spike is a single DNA molecule. Larger molecules elute first.
PicoSep RNA Integrity Analysis
Single molecule sensitivity enables detection of minute amounts of RNA degradation and more accurate quantification of RNA integrity by direct counting of degraded molecules.
PicoSep High MW DNA Sizing
PicoSep can accurately size and quantify high molecular weight DNA (100 kb+) in <2 hours. No electrophoresis method offers this speed, sizing resolution, or quantification capability.
PicoSep Single Molecule Conformation
Individual single molecule spikes can be analyzed to determine molecular information such as size, packing density, and conformation. This trace shows a large DNA molecule randomly folded at the leading front and stretched out at the rear.
Show More
 

Careers

We’re always looking for smart, dedicated, and creative people to join our team. As in any startup environment, candidates who are flexible enough to fill many roles can be expected to grow quickly within the company and play a large part in shaping its future. Today, you may be working at the lab bench while tomorrow, you may be writing a patent, and the day after, you may be meeting with customers.

Senior Scientist

Research Associate

About Us

Circulomics is a Maryland biotechnology company located within IMET's Harbor Launch Incubator. To date, Circulomics has received over $9M in funding from NIH and Maryland TEDCO to develop innovative platforms for nucleic acid sample preparation, long-read sequencing, nucleic acid assays, and single molecule analysis that will drive the research and clinical translation of genomics technologies from sequencing to expression profiling and biomarker analysis.

Circulomics Inc

701 E Pratt St, Baltimore, MD 21202

General Inquiries: info@circulomics.com

Orders and Sales Inquiries: orders@circulomics.com

Technical Support: support@circulomics.com

Business Development: busdev@circulomics.com

  • Twitter
  • LinkedIn Social Icon
  • Facebook
 
  • Grey Twitter Icon
  • Grey LinkedIn Icon
  • Grey Facebook Icon

701 E Pratt St, Baltimore, MD 21202

INFO@CIRCULOMICS.COM

PicoSep Single Molecule DNA Sizing

DNA molecules are individually detected (bottom) and counted to form chromatograms (top). Each vertical spike is a single DNA molecule. Larger molecules elute first.