Nanobind Support

Protocols​

 

Nanobind CBB Big DNA Kit - Handbook v1.8 (07/2019)

Extract high MW DNA (50 kb - 300 kb) from cultured mammalian cells, cultured bacteria (gram negative/positive), and whole blood. Extract ultra high MW DNA (50 kb - 1 Mb+) from cultured mammalian cells, cultured bacteria (gram negative), and whole blood. These protocols require the Nanobind CBB Big DNA Kit (NB-900-001-001).

Nanobind Plant Nuclei Big DNA Kit - Handbook v0.18 (10/2018)

Extract high MW DNA (50 kb - 300 kb) from plant nuclei. This protocol requires the Nanobind Plant Nuclei Big DNA Kit (NB-900-801-001).

Nanobind Tissue Big DNA Kit - Handbook v1 (11/2019)

Extract high MW DNA (50 kb - 300+ kb) from tissue samples. These protocols require the Nanobind Tissue Big DNA Kit (NB-900-701-001).

Nanobind Big DNA Kit - Version History (11/2019)

This document details all updates made to protocols and kits.

Automation Scripts​

 

Nanobind CBB Big DNA Kit - Automated Reference Guide v1.2 (03/2019) (Contact Us)

Extract high MW DNA (50 kb - 300 kb) from cultured mammalian cells, cultured bacteria (gram negative), and whole blood in less than 40 minutes. These protocols require the CBB chemistry and custom 3 mm Nanobind disks.

ThermoFisher KingFisher Duo Prime Big DNA Extraction Script -  GM12878 cells (Contact Us)

Automated extraction of high MW DNA (50 kb - 300 kb) from 5e6 GM12878 cells. This script requires the CBB chemistry which is available as a beta kit.

ThermoFisher KingFisher Duo Prime Big DNA Extraction Script - E. coli (Contact Us)

Automated extraction of high MW DNA (50 kb - 300 kb) from 1e9 E. coli cells. This script requires the CBB chemistry which is available as a beta kit.

ThermoFisher KingFisher Duo Prime Big DNA Extraction Script - Blood (Contact Us)

Automated extraction of high MW DNA (50 kb - 300 kb) from 200 uL Human Whole Blood. This script requires the CBB chemistry which is available as a beta kit.

Application Notes

cryoPREP Tissue Homogenization Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from tissue samples using Covaris cryoPREP for upfront tissue disruption. ONT GridION data is included.

Human Breast Application Note v1 (04/2020)

Describes the recommended protocol for extracting HMW from human breast tissue. ONT GridION data is included.

Mammalian Brain Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from mammalian brain tissue. ONT GridION data is included.

Mammalian Liver Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from mammalian liver tissue. PacBio Sequel I and ONT MinION data are included.

Mammalian Spleen Application Note v1 (04/2020)

Describes the recommended protocol for extracting HMW from mammalian spleen tissue. ONT MinION data is included.

Fish Testis Application Note v1 (04/2020)

Describes the recommended protocol for extracting HMW from fish testis. PacBio Sequel I and ONT GridION data are included.

Snail Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from black mystery snail. ONT MinION data is included.

Crab Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from crab skeletal muscle. PacBio Sequel II data is shown.

Fish Skeletal Muscle Application Note v1 (12/2019)

Describes the recommended protocol for extracting HMW from fish skeletal muscle and shrimp. PacBio Sequel II data included shown.

Nucleated Blood Application Note v1 (Coming Soon)

Describes the recommended protocol for extracting HMW DNA from nucleated blood samples from animals such as bird and fish. ONT PromethION data is shown.

Fish Tissue Sequencing Guide for ONT v1 (12/2019)

Describes the dissection, DNA extraction, and sequencing of nile tilapia on ONT GridION. Illustrates the effects of tissue type on sequencing read lengths and throughputs.

Aplysia Application Note v1 (12/2019)

Describes the dissection, DNA extraction, and sequencing of the sea slug Aplysia californica on PacBio Sequel II.

DNA Shearing Sequencing Guide v1 (Coming Soon)

Illustrates how the amount of DNA loaded onto a ONT

MinION flow cell affects read lengths and sequencing throughput.

DNA Loading Sequencing Guide  for ONT v1 (02/2020)

Illustrates how the amount of DNA loaded onto a ONT MinION flow cell affects read lengths and sequencing throughput.

SDS

BL3 Lysis/Binding Buffer

CLE3 Digestion Buffer

CT Tissue Disruption Buffer

CW1 Wash Buffer

CW2 Wash Buffer

EB Elution Buffer

PL1 Nuclei Lysis Buffer

PW1 Wash Buffer

Proteinase K

RNase A

SB Salting Out Buffer

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