Do You Want N50 Bragging Rights?
The Short Read Eliminator XL Kit uses size selective precipitation to dramatically reduce short reads below 40 kb.
Short DNA fragments <40 kb are progressively depleted (i.e. depletion efficiency increases as DNA gets shorter). Depletion range estimated based on sequencing read length distributions.
The read length enhancement is dependent on:
1) the amount of short DNA present in the input DNA
2) the amount of long DNA present in the input DNA
Read length N50 surpassing 50 kb has been achieved on Oxford Nanopore MinION/GridION/PromethION.
PacBio Sequel applications are coming soon.
Overall recovery efficiency is dependent on:
1) the quality of the input DNA
2) input DNA concentration.
Expect to recover 40-50% of your HMW DNA.
This kit requires very high quality HMW DNA (>>48 kb) or recovery efficiency will be compromised!
Processing requires only a simple centrifuge step and two wash steps. Total process time is 1 hour.
See our publications page for latest results.
1) SRE XL Short Read Eliminator Buffer - 1 mL and 2) EB Elution Buffer - 2 mL
Sufficient for at least 16 standard purifications.
Short Read Eliminator Kit - Handbook v2.0
DNA Loading Sequencing Guide for ONT v1
Short Read Eliminator Kit - Version History
Patents / Terms and Conditions
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To facilitate customs, Australian customers should provide an appropriate import permit.
We'd love to chat about sample prep and long-reads (firstname.lastname@example.org)!