The Short Read Eliminator XL Kit uses size selective precipitation to dramatically reduce short reads below 40 kb.
Short DNA fragments <40 kb are progressively depleted (i.e. depletion efficiency increases as DNA gets shorter). Depletion range estimated based on sequencing read length distributions.
The read length enhancement is dependent on:
1) the amount of short DNA present in the input DNA
2) the amount of long DNA present in the input DNA
Read length N50 surpassing 50 kb has been achieved on Oxford Nanopore MinION/GridION/PromethION.
PacBio Sequel applications are coming soon.
Overall recovery efficiency is dependent on:
1) the quality of the input DNA
2) input DNA concentration.
Expect to recover 40-50% of your HMW DNA.
This kit requires very high quality HMW DNA (>>48 kb) or recovery efficiency will be compromised!
Processing requires only a simple centrifuge step and two wash steps. Total process time is 1 hour.
