Short Read Eliminator Kit

SKU SS-100-101-01
$150.00
In stock
1
Product Details

Do You Hate Short Reads? So Do We!

Our Short Read Eliminator Kit uses size selective precipitation to dramatically reduce short reads below 25 kb.

Short DNA fragments <25 kb are progressively depleted (i.e. depletion efficiency increases as DNA gets shorter). DNA <10 kb are nearly completely removed. Depletion range estimated based on sequencing read length distributions and gel electrophoresis.


The read length enhancement is dependent on:

1) the amount of short DNA present in the input DNA

2) the amount of long DNA present in the input DNA

Read length N50 is increased by up to 25 kb on Oxford Nanopore MinION/GridION/PromethION.

PacBio Sequel applications are coming soon.


Overall recovery efficiency is dependent on:

1) the quality of the input DNA

2) the input DNA concentration.

Expect to recover 50-70% of your HMW DNA.

Sample must be predominantly HMW DNA (>48 kb) or recovery efficiency will be compromised.


Processing requires only a simple centrifuge step and two wash steps. Total process time is 1 hour.


See our publications page for latest results.


Includes:

1) SRE Short Read Eliminator Buffer - 1 mL and 2) EB Elution Buffer - 2 mL

Sufficient for at least 16 standard purifications.


Protocols

Short Read Eliminator Kit - Handbook v2.0


Application Notes


Version History

Short Read Eliminator Kit - Version History


Safety

SDS


Legal

Patents / Terms and Conditions


International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.


We'd love to chat about sample prep and long-reads (info@circulomics.com)!

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701 E Pratt St, Baltimore, MD 21202

INFO@CIRCULOMICS.COM

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