SRE kit

The SRE kit uses size-selective precipitation to dramatically reduce short reads below 10 kb.

Short DNA fragments <25 kb are progressively depleted (i.e., depletion efficiency increases as DNA gets shorter). DNA <10 kb are nearly completely removed. Depletion range estimated based on sequencing read length distributions and gel electrophoresis.

The read length enhancement is dependent on:

1. the amount of short DNA present in the input DNA

2. the amount of long DNA present in the input DNA

Overall recovery efficiency is dependent on:

1. the quality of the input DNA

2. the input DNA concentration.

Expect to recover 50–70% of your HMW DNA.

Sample must be predominantly HMW DNA (>30 kb) or recovery efficiency will be compromised.

Processing requires only a simple centrifuge step and two wash steps. Total process time is two hours with 10 min hands on time.

Includes:

1. Buffer SRE — 1 mL

2. Buffer EB — 5 mL

Sufficient for at least 16 standard purifications.

New procedure and checklist optimized for higher yield: https://www.pacb.com/wp-content/uploads/Procedure-checklist-Removing-short-DNA-fragments-with-the-Short-Read-Eliminator-SRE-kit.pdf

Safety

SDS

All kits are guaranteed for one year from the date of purchase.

Legal

Patents / Terms and conditions

International customers

Additional import duties/taxes/fees (i.e., VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.

We’d love to chat about sample prep and long-reads (support@pacb.com)