Reagents to complete depletion/size selection of DNA <10 kb
The SRE XS kit uses size-selective precipitation to dramatically reduce short reads below 10 kb.
This kit is more tolerant of fragmented DNA and low DNA concentration than its siblings. It also features a higher recovery efficiency (50–90%).
Short DNA fragments <10 kb are progressively depleted (i.e., depletion efficiency increases as DNA gets shorter). DNA <4 kb are nearly completely removed. Depletion range estimated based on sequencing read length distributions and gel electrophoresis.
The read length enhancement is dependent on:
1. the amount of short DNA present in the input DNA
2. the amount of long DNA present in the input DNA
This kit has been tested on Oxford Nanopore MinION/GridION/PromethION.
PacBio Sequel applications are coming soon.
Overall recovery efficiency is dependent on:
1. the quality of the input DNA
2. input DNA concentration
Expect to recover 50–90% of your HMW DNA.
This kit can be used with HMW DNA, fragmented DNA, sheared DNA, and PCR amplicons.
Processing requires only a simple centrifuge step and two wash steps. Total process time is one hour.
1. Buffer SRE XS — 1 mL
2. Buffer EB — 5 mL
Sufficient for at least 16 standard purifications.