The SRE XL kit uses size-selective precipitation to dramatically reduce short reads below 40 kb.
Short DNA fragments <40 kb are progressively depleted (i.e., depletion efficiency increases as DNA gets shorter). Depletion range estimated based on sequencing read length distributions.
The read length enhancement is dependent on:
1. the amount of short DNA present in the input DNA
2. the amount of long DNA present in the input DNA
Overall recovery efficiency is dependent on:
1. the quality of the input DNA
2. input DNA concentration.
Expect to recover 40–50% of your HMW DNA.
This kit requires very high quality HMW DNA (>48 kb) or recovery efficiency will be compromised
Processing requires only a simple centrifuge step and two wash steps. Total process time is one hour.
1. Buffer SRE XL — 1 mL
2. Buffer EB — 5 mL
Sufficient for at least 16 standard purifications.
Procedure and checklist
All kits are guaranteed for one year from the date of purchase.
Patents / Terms and conditions
Additional import duties/taxes/fees (i.e., VAT or GST where appropriate) will be payable directly to the import broker FedEx.
To facilitate customs, Australian customers should provide an appropriate import permit.
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