Nanobind plant nuclei kit

The Nanobind plant nuclei kit is designed for rapid extraction of large DNA from plant nuclei.

The Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing.

Nanobind HMW DNA is optimized for HiFi sequencing.

First, nuclei are isolated from 1–5 g of plant tissue using one of the recommended nuclei isolation protocols. Then, HMW DNA is extracted from the nuclei using Nanobind disks. Each of the two purification steps (i.e., nuclei isolation + Nanobind extraction) removes different contaminants from the sample, resulting in clean, HMW DNA from even the most challenging plant species.

It's been internally tested on an array of plant species using PacBio sequencing. In addition, customers have sequenced a growing variety of plants such as lettuce, tomato, peppers, brassica, lavender, wheat, celery, grape, and maize.

For these plant types, HMW DNA (up to 300+ kb) can be obtained in <1 hour from the nuclei pellet stage.

Typical yields are 5–20+ µg per extraction, depending on input

See our publications page for latest results.

Includes:

The Nanobind plant nuclei kit is comprised of the Nanobind plant nuclei kit RT (102-207-800) and Nanobind plant nuclei kit 4C (102-207-900). Components from both kits are required to complete HMW DNA extraction from plant nuclei (24 reactions per kit).

Guide and overview

Nanobind plant nuclei kit

See Nanobind support page for full list.

Procedure and checklist

Plant nuclei

Safety

SDS

All kits are guaranteed for one year from the date of purchase.

Legal

Patents / Terms and conditions

International customers

Additional import duties/taxes/fees (i.e., VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.

We'd love to chat about sample prep and long-reads (support@pacb.com)