HMW DNA extraction from plant nuclei (24 reactions)
The Nanobind plant nuclei kit is designed for rapid extraction of large DNA from plant nuclei.
The Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.
First, nuclei are isolated from 1–5 g of plant tissue using one of the recommended nuclei isolation protocols. Then, HMW DNA is extracted from the nuclei using Nanobind disks. Each of the two purification steps (i.e., nuclei isolation + Nanobind extraction) removes different contaminants from the sample, resulting in clean, HMW DNA from even the most challenging plant species.
It's been internally tested on an array of plant species using Oxford Nanopore and PacBio sequencing. In addition, customers have sequenced a growing variety of plants such as lettuce, tomato, peppers, brassica, lavender, wheat, celery, grape, and maize.
For these plant types, big DNA (up to 300+ kb) can be obtained in <1 hour from the nuclei pellet stage.
Typical yields are 5–20+ µg per extraction, depending on input
The Nanobind plant nuclei kit is comprised of the Nanobind plant nuclei kit RT (102-207-800) and Nanobind plant nuclei kit 4C (102-207-900). Components from both kits are required to complete HMW DNA extraction from plant nuclei (24 reactions per kit).