The Nanobind Plant Nuclei Big DNA Kit is designed for rapid extraction of really big DNA from plant nuclei.
Our Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.
It's been internally tested on an array of plant species using Oxford Nanopore and PacBio sequencing. In addition, customers have sequenced a growing variety of plants such as lettuce, tomato, peppers, brassica, lavender, wheat, celery, grape, and maize.
For these plant types, big DNA (up to 300+ kb) can be obtained in <1 hour from the nuclei pellet stage.
Typical yields are 5 ug - 20+ ug per extraction depending on input
Users will need to perform nuclei isolation using their own protocols or one of the suggested protocols such as Workman et al. Protocol Exchange (2018) DOI:10.1038/protex.2018.059 or Zhang et al. Nature Protocols (2012) DOI:10.1038/nprot.2011.455.