Nanobind Plant Nuclei Big DNA Kit

SKU NB-900-801-01
$400.00
HMW DNA From Plant Nuclei
In stock
1
Product Details

Extracting from Plants?

The Nanobind Plant Nuclei Big DNA Kit is designed for rapid extraction of really big DNA from plant nuclei.

Our Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.

First, nuclei are isolated from 1–5 g of plant tissue using one of the recommended nuclei isolation protocols. Then, HMW DNA is extracted from the nuclei using Nanobind disks. Each of the two purification steps (i.e. nuclei isolation + Nanobind extraction) removes different contaminants from the sample, resulting in clean, HMW DNA from even the most challenging plant species.

It's been internally tested on an array of plant species using Oxford Nanopore and PacBio sequencing. In addition, customers have sequenced a growing variety of plants such as lettuce, tomato, peppers, brassica, lavender, wheat, celery, grape, and maize.

For these plant types, big DNA (up to 300+ kb) can be obtained in <1 hour from the nuclei pellet stage.

Typical yields are 5 ug - 20+ ug per extraction depending on input

See our publications page for latest results.

We will make every effort to help you get big DNA!


Includes:

1) Proteinase K, 2) RNase A, 3) PL1 Nuclei Lysis Buffer, 4) PW1 Wash Buffer, 5) EB Elution Buffer, and 6) 20x MagNanobind Disks.

20 extractions per kit.


Protocols

Nanobind Plant Nuclei Big DNA Kit Handbook

See Nanobind Support page for full list.



Safety

SDS

All Kits are Guaranteed for 1 Year from the Date of Purchase.


Legal

Patents / Terms and Conditions


International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.


We'd love to chat about sample prep and long-reads (info@circulomics.com)!

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