HMW DNA extraction from cells, bacteria, and blood (96 reactions) for automation only.
The Nanobind HT CBB kit is design for high-throughput (HT) high molecular weight (HMW) DNA extraction from mammalian cell and 200 ul whole human blood. Nanobind extracted DNA is optimized for HiFi sequencing.
The kit uses Nanobind magnetic disk processing to automate lysis, binding, washing and elution steps and is compatible with instruments from Hamilton and Thermo Fisher.
We are providing scripts for mammalian cell and human blood on the following instruments:
Hamilton NIMBUS Presto, fully automated workflow and walk away solution processing 24-96 samples per run in ~ 2 hrs 30 min.
Thermo Fisher KingFisher Apex, Flex, and Duo Prime semi-automated systems with manual plate filing and one user intervention during run. The Duo Prime system processes 6-12 samples per run in <2 hrs and the Apex / Flex systems process 24-96 samples in ~ 2 hrs
Typical yield are: 3–12 μg for human blood (for donor WBC count ≥4 X 106 cells/mL) and 5–15 μg for mammalian cells (for 106 cells).
The Nanobind HT CBB kit is comprised of the Nanobind CBB kit RT (102-762-900) and Nanobind CBB kit 4C (102-763-000). Components from both kits are required to complete HMW DNA extraction from mammalian cells and 200 μl blood (96 reactions per kit).