Nanobind CBB Big DNA Kit

Want Long Reads and High Sequencing Yields?

The Nanobind CBB Big DNA Kit is designed for rapid extraction of really big DNA from cultured cells, cultured bacteria (gram negative and gram positive), and whole blood (nucleated and non-nucleated).

Our Nanobind magnetic disks use nanostructured silica to protect DNA from shearing and facilitate washing to generate the largest and cleanest DNA for long-read sequencing and optical mapping.

This kit has been used to generate the longest sequencing data ever and can generate reads in excess of 4 Mb in length. Read length N50 of 30 - 50 kb can be expected in standard sequencing applications but N50 in excess of 150 kb can be achieved using ultra long sequencing protocols.

Combine with our Short Read Eliminator Kits to further enhance reads lengths.

Nanobind HMW DNA works well on platforms including: 1) PacBio RSII/Sequel, 2) Oxford Nanopore MinION/GridION/PromethION, 3) Bionano Genomics Irys/Saphyr, 4) 10X Genomics Chromium and 5) any other application high quality DNA is needed.

Includes protocols for HMW DNA (50 kb - 300+ kb) and UHMW DNA (50 kb - 1 Mb+) extraction.

Typical yields are 5 ug - 100+ ug per extraction depending on input.

This kit has not been validated for complex sample types.

A high-throughput version of this kit is available for automated extractions on Thermo Fisher KingFisher instruments. Contact us for details.

See our publications page for latest results.

We will make every effort to help you get big DNA!

Includes:

1) Proteinase K, 2) RNase A, 3) CLE3 Digestion Buffer, 4) BL3 Lysis/Binding Buffer, 5) CW1 Wash Buffer, 6) CW2 Wash Buffer, 7) EB Elution Buffer, and 8) 20x Nanobind Disks.

20 extractions per kit.

Gram positive bacteria requires user supplied lysozyme and STET buffer.

Protocols

Nanobind CBB Big DNA Kit Handbook

See Nanobind Support page for full list.

Application Notes

DNA Loading Sequencing Guide for ONT v1

Safety

SDS

All Kits are Guaranteed for 1 Year from the Date of Purchase.

Legal

Patents / Terms and Conditions

International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.

We'd love to chat about sample prep and long-reads (support@pacb.com)!