Nanobind CBB Big DNA Kit (Cells, Bacteria, Blood)

SKU NB-900-001-01
$400.00
In stock
1
Product Details

Want Long Reads and High Sequencing Yields?

The Nanobind CBB Big DNA Kit is designed for rapid extraction of really big DNA from cultured cells, cultured bacteria (gram negative and gram positive), and whole blood (nucleated and non-nucleated).

This kit has been used to generate the longest sequencing data ever and can generate reads in excess of 2 Mb in length. Read length N50 of 30 - 50 kb can be expected in standard sequencing applications but N50 in excess of 150 kb can be achieved using ultra long sequencing protocols.

Combine with our Short Read Eliminator Kits to further enhance reads lengths.

Nanobind HMW DNA works well on platforms including: 1) PacBio RSII/Sequel, 2) Oxford Nanopore MinION/GridION/PromethION, 3) Bionano Genomics Irys/Saphyr, 4) 10X Genomics Chromium and 5) any other application high quality DNA is needed.

Includes protocols for high MW DNA (50 kb - 300+ kb) and ultra high MW DNA (50 kb - 1 Mb+) extraction.

Typical yields are 5 ug - 100+ ug per extraction depending on input.

This kit has not been validated for complex sample types.

A high-throughput version of this kit is available for automated extractions on Thermo Fisher KingFisher instruments. Contact us for details.

See our publications page for latest results.

We will make every effort to help you get big DNA!


Includes:

1) Proteinase K, 2) RNase A, 3) CLE3 Digestion Buffer, 4) BL3 Lysis/Binding Buffer, 5) CW1 Wash Buffer, 6) CW2 Wash Buffer, 7) EB Elution Buffer, and 8) 20x MagNanobind Disks.

20 extractions per kit.

Gram positive bacteria requires user supplied lysozyme and STET buffer.


Protocols

Nanobind CBB Big DNA Kit - Handbook v1.8


Application Notes

DNA Loading Sequencing Guide for ONT v1


Version History

Nanobind Big DNA Kit - Version History


Safety

SDS


Legal

Patents / Terms and Conditions


International Customers

Additional import duties/taxes/fees (i.e. VAT or GST where appropriate) will be payable directly to the import broker FedEx.

To facilitate customs, Australian customers should provide an appropriate import permit.


We'd love to chat about sample prep and long-reads (info@circulomics.com)!

Save this product for later

701 E Pratt St, Baltimore, MD 21202

INFO@CIRCULOMICS.COM

  • Grey Twitter Icon
  • Grey LinkedIn Icon
  • Grey Facebook Icon